Alves, Fábio de Matos , Sforça, Danilo Augusto , Fortuna Perez , Ana Paula , Sartori, Ângela Lúcia Bagnatori , Souza, Anete Pereira de .
Development and characterization of a DNA library enriched on Microsatellite (SSR) of Prosopis ruscifolia Griseb.
Microsatellites or simple sequence repeats (SSR) markers are a valuable tool in genetic mapping, genotyping, analysis of paternity, gene flow and marker-assisted selection in breeding due to their characterizationof co-dominant loci, high allelic variation and be easily used by PCR. Data on microsatellite abundance comes preferentially from microsatellite enriched libraries and DNA sequence databases. This study aimed to develop and characterize a microsatellite-enriched genomic library of Prosopis ruscifolia Griseb. and subsequent synthesis and evaluation of primers. The construction of this library enriched of SSR was done using leaves from one individual of P. ruscifolia, collected in Porto Murtinho,Mato Grosso do Sul, Brazil. As a consequence, 95 clones were generated and subsequently sequenced, resulting in 31 sequences among contigs and singlets; besides 38 perfect and imperfect microsatellites were sampled. In this study, 18 motifs dinucleotides, 9 motifs AC/AC, 8 motifs GT/TG, one motif GA, one motif trinucleotide (CTT), and one motif tetranucleotide (GCTT) were totalized. The remaining sequences presented motifs with compounds microsatellites.The primers designed of this library are in the process of synthesis and it will generate data for biodiversity studies of Prosopis ruscifolia.
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1 - Universidade Estadual de Campinas, Centro de Biologia Molecular e Engenharia Genética, Av. Cândido Rondon, 400, Barão Geraldo, Campinas, São Paulo, 13083-875, Brazil
2 - Universidade Estadual de Campinas, Biologia Vegetal, Monteiro Lobato, 970 - Barão Geraldo - , Campinas, São Paulo, 6109, Brazil
3 - Universidade Federal de Mato Grosso do Sul, CCBS, Botânica, Cidade Universitária, Campo Grande, Mato Grosso do Sul, 549, Brazil
Presentation Type: Poster:Posters for Topics
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM