Recent Topics Posters
Moore, Michael , Gitzendanner, Matthew , Alverson, Andrew , Harris, Robert , Soltis, Pamela , Soltis, Douglas .
Exploring plastid and mitochondrial genome coverage for 12 basal angiosperms multiplexed in a single Illumina lane.
Massively parallel sequencing (MPS) platforms provide a rich source of characters for refining our understanding of plant evolutionary relationships. To date, most studies of plant phylogeny using MPS data have focused on plastid genome data due to the structural and evolutionary simplicity of this genome, and because substantial plastid genome data have already been generated for numerous taxa. In fact, it is usually possible to obtain substantial or even complete coverage of the plastid genome when sequencing genomic DNA using MPS technology, even when multiplexing on a single lane, because plastid DNA comprises a disproportionately large portion of the total genomic DNA in a cell (~5%). Substantial mitochondrial sequence is also typically generated via MPS sequencing of genomic DNA for the same reason. The actual percent and depth of coverage for organellar genomes on MPS platforms may be controlled by a number of variables including the number of genomic DNAs multiplexed per lane and the size of the nuclear genome, as well as instrument-specific factors such as read length and number of reads generated per lane. To explore the yield of organellar sequence, we multiplexed total genomic DNA libraries for 12 diverse basal angiosperm taxa with a wide range of nuclear genome sizes in a single lane of an Illumina GAII, using 100 bp single-end reads. We report statistics on coverage for both plastid and mitochondrial assemblies, and discuss the importance of a multiplex genomic DNA sequencing approach for generating dual, independent organellar genome estimates of plant phylogeny.
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Moore lab website
1 - Oberlin College, Department of Biology, 119 Woodland St., Oberlin, OH, 44074, USA
2 - University Of Florida, Florida Museum Of Natural History, PO BOX 117800, GAINSVILLE, FL, 32611-7800, USA
3 - Indiana University, Department of Biology, 1001 E. 3rd St., Bloomington, IN, 47405, USA
4 - Florida Museum of Natural History, Dickinson Hall, Gainesville, FL, 32611, USA
5 - University Of Florida, Florida Museum Of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA
6 - University of Florida, Department of Biology, PO Box 117800, Gainesville, FL, 32611, USA
Next Generation Sequencing
Presentation Type: Recent Topics Poster
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM