Recent Topics Posters
Garza, Rosario , Tumonong, May , Johnson, Brittany , Cohen, Jim .
The Utility of Mitochondrial DNA Regions for Phylogeny Reconstruction in Boraginaceae.
The plant mitochondrial genome is used only infrequently for phylogeny reconstruction. This is due to the fact that mitochondrial DNA (mtDNA) tends to have the slowest mutation rate of the three plant genomes (chloroplast, nuclear, and mitochondria) as well as a high rate of recombination. Despite these drawbacks, the mitochondrial genome provides an appealing pool of DNA regions for phylogeny reconstruction in Boraginaceae, and relationships resolved from these regions can provide insights into evolutionary patterns different from those derived from the chloroplast and nucleus. In this study on Boraginaceae, ten different mtDNA regions were screened; of these, the nad5/1-nad5/2 intergenic region was most easily amplified and sequenced. Sequences from 33 species from throughout the family were obtained for this mtDNA region, and these sequences were aligned and subsequently analyzed in a maximum parsimony (MP) framework. The resulting phylogeny is compared to that of ndhF, a coding chloroplast DNA (cpDNA) region. The phylogeny of nad5/1-nad5/2 has much less resolution than that of ndhF, although three tribes, Boragineae, Cynoglosseae, and Trichodesmeae, are resolved by analyses of the mtDNA region. In general, analyses of nad5/1-nad5/2 and those of ndhF resulted in similar relationships, with one exception. The mtDNA region resolved Lappula as sister to Myosotis, but this relationship was not recovered in analyses of the cpDNA region. An unexpected finding is that across all species of Boraginaceae, the rate of evolution in nad5/1-nad5/2 was much greater among species of Cynoglosseae than in other groups. In contrast, ndhF has an equal rate of evolution among species of the family.
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2 - texas a&m international univesity, Biology and Chemistry, 5201 University Blvd, Laredo, TX, 78041, USA
mitochondrial DNA Sampling
Presentation Type: Recent Topics Poster
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM