Abstract Detail

Recent Topics Posters

Pérez-Torres, Claudia-Anahí [1], Mendez-Bravo, Alfonso [2], Ibarra-Laclette, Enrique [3], Hernandez-Guzman, Gustavo [3], Lopez-Gomez, Rodolfo [4], Herrera-Estrella, Luis [5].

Avocado genome sequencing project.

Avocado (Perseaamericana) is a major fruit crop with high nutritional and industrial value. Itis one of the rare crop plants among the basal angiosperms that belong to Lauracea family. Genomic characterization of avocado will provide anopportunity to examine the developmental genetics of fleshy fruits and some ofthe mechanisms of early evolutive adaptations in flowering plants. To generate de novo draft genome sequences from avocado genome we combined several massive sequencing strategies: conventional BACs paired-end (PE) Sanger sequencing with ABI 3730xl sequencer; single-end (SE) and PE 454 reads from Roche GS platforms, and PE reads from SOLiD sequencer. First, we constructed a BACs library, from which we generated 55,824 Sanger Pair-End sequences (approximately 0.03-foldgenome sequence coverage). We then used sheared genomic DNA to generate 34 readsets on Roche GS-Titanium and GS-Plus sequencers, producing about forty three million reads with an average length of 338.84 and 583.87 bp respectively (16.1-fold genome sequence coverage). We supplemented these data sets with about six million pair-end 454 reads (approximately 2-fold genome sequence coverage; sequencing libraries were constructed with insert sizes of 3 and 8kbp). Finally, we generated around 125 million short PE reads by using the SOLiD system (approximately 6.2 Gpb of sequence data; 6.4-fold genome sequence coverage). In total our reads collection represent 24.5-fold redundant coverage of the Persea americana var. drymifolia genome. In the near future, we expect to conclude the full genome assembly and gene annotation through integrating both, transcriptomic and genomic sequences. In this poster I will present the advances in the transcritome analysis and the progress of genome assembly.

Broader Impacts:

1 - Langebio-Cinvestav Campus Guanajuato, Fisiologia e Ingenieria Metabolica de Plantas, Km. 9.6 Libramiento Norte Carretera Irapuato-Leon, Irapuato, Gto, 36821
2 - Langebio-Cinvestav Campus Guanajuato, Fisiologia e Ingenieria Metabolica de Plantas, Km. 9.6 Libramiento Norte Carretera Irapuato-Leon, Irapuato, Gto., 36821
3 - LANGEBIO-CINVESTAV Campus Guanajuato, Unidad de Servicios Genómicos, Km. 9.6 Libramiento Norte Carretera Irapuato-León, Irapuato, Gto., 36821
4 - Universidad Michoacana de San Nicolas de Hidalgo, Av. Fco. J. Múgica S/N., Morelia, Michoacan, 58030
5 - Langebio-Cinvestav Campus Guanajuato, Fisiologia eIngenieria Metabolica de Plantas, Km. 9.6 Libramiento Norte Carretera Irapuato-León, Irapuato, Gto., 36821

Keywords:
none specified

Presentation Type: Recent Topics Poster
Session: P
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM
Number: PRT033
Abstract ID:1099