Evolutionary Developmental Biology (Evo-Devo)
Gleissberg, Stefan , Hidalgo, Oriane , Bartholmes, Conny .
Roles of a leaf polarity gene in dissected leaf development.
Dissected leaves produce lateral structures such as leaflets from a marginal blastozone during primary morphogenesis. These primordial margins form along a contact layer of adaxial and abaxial identities, hence leaf dissection relies on the proper establishment of bifacial leaf structure. Medio-lateral expansion of leaf blades and tissue maturation during secondary morphogenesis also occur along this boundary of adaxial and abaxial domains. Studies in species with dissected leaves have revealed several important genetic components of leaf dissection, whereas polarity establishment has been studied mostly separately in species with simple leaves. Here, we investigate the disruption of a leaf polarity pathway in the contex of dissected leaf development, using virus-induced gene silencing (VIGS) in Eschscholzia californica. We have characterized five YABBY genes in Eschscholzia that belong to three angiosperm-wide gene clades known to act in leaf development downstream of leaf polarity pathways. We show that Eschscholzia YABBY gene expression is associated with leaflets, and that VIGS-mediated downregulation results in defects in leaf polarity and primordial margin establishment, impacting bifaciality, leaflet initiation, and leaf architecture.
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1 - Ohio University, Environmental and Plant Biology, Porter Hall 500, Athens, Ohio, 45701, USA
Presentation Type: Oral Paper:Papers for Topics
Location: Lindell D/Chase Park Plaza
Date: Wednesday, July 13th, 2011
Time: 10:45 AM