Abstract Detail

Developmental and Structural Section

Brauer, Laura [1], Carlsward, Barbara [2].

Rehydration Techniques for Dried Leaf Tissue of Badiera (Polygalaceae).

Leaf samples were treated in boiling water with Aerosol OT surfactant, 3% potassium hydroxide, and 14.8 N ammonium hydroxide. All specimens were then washed and stored in 70% ethyl alcohol. For paraffin processing, specimens were dehydrated in a graduated tertiary butyl alcohol/ethyl alcohol series, embedded in paraffin, and sectioned at 10 µm on a rotary microtome. Sections were stained with Heidenhain's iron-alum hematoxylin and safranin and mounted on slides with Permount. Potassium hydroxide treatments removed all protoplasmic contents but generally returned cells to full turgor. Water with Aerosol OT did not remove cell contents but resulted the most distorted mesophyll. Ammonium hydroxide treatment not only preserved cell contents but also returned cells to full turgor. While water with Aerosol OT is the most cost effective and least time consuming, ammonium hydroxide gives the best overall results and is the least cost effective and most time consuming.

Broader Impacts:

1 - Eastern Illinois University, 600 Lincoln Avenue, Charleston, IL, 61920, USA
2 - Eastern Illinois University, Biological Sciences, 600 Lincoln Avenue, Charleston, IL, 61920, USA

Keywords:
rehydration
potassium hydroxide
ammonium hydroxide
leaf
mesophyll
Aerosol OT.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM
Number: PDS001
Abstract ID:177