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Abstract Detail

Developmental and Structural Section

Dotzek, Jana [1].

Putative mitochondria-mediated speciation barriers in Oenothera - An example for recombination between mitochondrial genomes in sexual crosses?

The genus Oenothera (evening primrose) has an outstanding scientific tradition and was developed as model for cytoplasmatic inheritance and speciation over the past century. Due to biparental transmission of plastid genomes within intra-specific crosses of Oenothera species, frequently so called plastome-genome incompatibility or plastid-mediated incompatibility, is observed. These incompatibilities are speciation barriers under the Dobzhansky-Muller model of speciation. However,besides the plastid-mediated there are also other incompatibilities described in the genus, which are independent from plastid, as well as from nuclear inheritance. They point to a biparental inheritance of a further cytoplasmatic component. One of these incompatibilities is the so-called falcifolia-syndrome, which arises in reciprocal crosses between strains of Oe.glazioviana and Oe. biennis with different frequency, depending on the mixing ratio of the involved cytoplasms. Since plants which inherit their plastid genomes biparental often also display biparental inheritance or paternal leakage of mitochondrial genomes, it is reasonable to assume the mitochondrial genome as genetic base for the falcifolia-syndrome. This would be the first case of a hybridization barrier, which is mediated alone by the mitochondria genome, probably due to recombination between the two mitochondria genomes involved. To address this question we established a protocol for mitochondria isolation in the biochemically challenging genus Oenothera and obtained pure mtDNA for next generation sequencing approaches. So far we did a comparative sequencing of seven mitochondrial genomes from different Oenothera species, including lines which display the falcifolia-syndrome in sexual crosses. First studies on the inheritance of the mitochondrial genome in Oenothera will be presented.

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1 - Max-Planck-Institute of Molecular Plant Physiology, Potsdam–Golm, Germany

Oenothera mitochondria comparative sequencing.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM
Number: PDS006
Abstract ID:275

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