Schwartz, Emily , Neubig, Kurt , Judd, Walter , Blanchard, Orland .
Molecular and morphological phylogenetic study of species delimitation in Kosteletzkya depressa and K. hispidula (Malvaceae, Hibisceae).
Ongoing phylogenetic studies of the genus Kosteletzkya have revealed unresolved phylogenetic relationships among K. depressa and K. hispidula. In this study, multiple population accessions from throughout the two species distributions were sampled in order to run analyses using molecular and morphological data. Two plastid loci (trnL-F and rpl32-trnL) and two nuclear loci (GBSSI and CesA1) were sequenced for all accessions of K. depressa and K. hispidula. Eleven morphological characters were chosen for analysis and character states were scored for each accession. In the combined analysis of all four gene regions, K. hispidula is strongly supported as a monophyletic clade. Kosteletzkya depressa appears as a paraphyletic group relative to the K. hispidula clade, and, according to DNA data alone, K. hispidula is sister to the NW Mexican K. depressa clade. In a combined analysis of the four gene regions and morphology, the K. hispidula clade is reinforced as a strong monophyletic group. However, a clade consisting of K. hispidula and K. ramosa appears to be sister to K. depressa, the populations of which also form a clade. This discrepancy could be explained by introgressive hybridization between K. hispidula and K. depressa resulting in a chloroplast capture event. A shared plastid haplotype, seen in rpl32-trnL, may be a molecular remnant of a chloroplast donation from K. hispidula to NW K. depressa populations (or vice versa) in the sympatric region of northwest Mexico, and is an incomplete and misleading representation of their true phylogenetic relationship. The results of the study exhibit the need for further research, with additional gene loci (both nuclear and plastid), in order to increase our confidence in the evolutionary history of these plants.
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1 - University of Florida, Department of Biology, Po Box 118526, Gainesville, FL, 32611, USA
2 - University of Florida, Florida Museum of Natural History, PO Box 117800, Gainesville, FL, 32611, USA
Presentation Type: Poster:Posters for Sections
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM