Abstract Detail

Advances in Plant Systematics and Population Genomics: Applications of Next Generation Techniques

Udall, Joshua [1], Bajgain, Prabin [1], Richardson, Bryce [2].

Natural sequence diversity of Artemesia tridentata in the western United States.

Artemisia tridentata (big sagebrush) is one of the most widely distributed and ecologically important shrub species in western Northern America. This species serves as a critical habitat and food resource for many animals and invertebrates. Habitat loss due to disturbances, usually wildfire, followed by establishment of invasive plant species is a serious threat to big sagebrush ecosystem sustainability. Taxonomic relationships within this species remain in question. Based on morphological, ploidy and chemical tests, big sagebrush is divided into three widespread subspecies.However, previous studies have shown that hybridization could be common and distinguishing subspecies based on the characters mentioned above can be problematic. Using 454 sequencing, we recently generated the first set ESTs forthis species. Partial gene sequences (~360 bp) from 48 genes were targeted for re-sequencing from individuals across the range of big sagebrush (~350individuals, 11 western states). Amplicons were generated using Fluidigm AccessArray and they were sequenced using 454. Based on these sequences, we present insight into the underlying phylogeographic and population genetic structure of big sagebrush.

Broader Impacts:

1 - Brigham Young University, Plant and Animal Science Dept, 292 Widstoe Building, Provo, UT, 84602, USA
2 - USDA Forest Service, Rocky Mountain Research Station, 735 North 500 East, Provo, Utah, 84606, USA

Keywords:
Sagebrush
454 sequencing
Fluidigm
Amplicon
population genetics.

Presentation Type: Symposium or Colloquium Presentation
Session: SY03
Location: Lenox Room/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 1:45 PM
Number: SY03002
Abstract ID:330