Mahady , Gail B , Soni, Kapil K , Locklear, Tracie , Patel, Udeshi .
In vitro and in vivo effects of Ayurvedic traditional medicines for the management of asthma.
The medicinal plants Bacopa monnieri (Brahmi, whole plant)and Boswellia serrata and Osimum sanctum (Holy basil) were collected from Vidisha (M.P.), India based on literature and traditional use in Ayurvedia to treat asthma. Materials were dried, pulverized to powder and extracted in methanol to exhaustion. The methanol extracts were tested in three in vitro assays, leukotriene-C4-synthase, leukotriene-A4-hydroxylase and cyclooxygenase-2. The active extracts were partitioned in dichloromethane(DCM), ethyl acetate (EtoAc) and 50% methanol and each partition was re-tested for activity. Extracts and partitions of the three plant species showed inhibition of all three enzymes in vitro suggesting anti-inflammatory and anti-asthmatic activities. Dried extracts of all three plants were tested in 24 BALB/c mice (6arms, 4 animals per arm) sensitized by intaperitoneal injections of OVA weekly for 3 weeks and then treated by gastric lavage for four days with the extracts at a dose of 100 mg/kg for four days plus intranasal OVA challenge. Control animals were treated with PBS (negative control) or dexamethasone (positive control treatment). Animals were subjected to 2-D in vivo imaging using a Xenogen IVIS 2000 (Caliper Corp) system and lung inflammation was assessed based on the treatments. Animals sensitized and challenged with OVA had significant lung and peritoneal inflammation as compared with the PBS control group. Treatment of the mice with dexamethasone reduced OVA-induced inflammation by 50-60%. Treatment of the mice with the Ayurvedic herbal extracts also reduced inflammation, with the activities of the extracts being in the order of Ocimum sanctum>Boswellia serrata>Bacopa monnieri. These data support the use of these Ayurvedic herbs for the management of the inflammatory symptoms of inflammatory asthma.
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1 - University of Illinois at Chicago, Pharmacy Practice, 833 S Wood St, Chicago, IL, 60612, USA
Presentation Type: Poster:Posters for Sections
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM