Panes, Vivian .
Identification and Cloning of Ribosome Inactivating Protein (RIP) Genes in Selected Species of the Euphorbiaceae.
Ribosome-inactivating proteins (RIP) inhibit protein synthesis in eukaryotic cells by catalytically damaging ribosomes. They have been proven to have an antiviral and antitumour activity because RIPs can truncate the translation of viral coat proteins. The presence of RIP genes in the 13 selected Euphorbiaceae species was screened through genomic DNA PCR using 5 sets of primers. The selected plants included Acalypha hispida Burm., Codiaeum variegatum L. Bl., Euphorbia hirta, Euphorbia lactea Haw., Euphorbia milii, Euphorbia pulcherrima Willd., Euphorbia tirucalli L., Euphorbia trigona Haw., Exoecaria cochinensis var. purpurascens, Jatropha pandorifolia Andr., Jatropha podagrica Hook, Manihot esculenta Crantz and Pedialanthus tithymaloides L. (Jatropha curcas L. was used as the control because it has already a known RIP gene as determined by Lin et al. 2003b). The genomic DNA sequences obtained were analyzed through BLASTs and ClustaW of MEGA4. PCR products were also cloned and sequenced. RIP primers generated PCR products for only three plants. These are J. podagrica, J. pandorifolia and J. curcas. Sequence analysis and alignment through BLAST showed 87%-90% homology of J. pandurifolia's RIP gene sequence to J. curcas precursor gene and mRNA,while 87%-89% homology of J. podagrica's RIP gene sequence to J. curcas' curcin precursor mRNA and gene. Nucleotide sequence and deduced amino acid sequence through Clustal W revealed possible ORFs in J. podagrica and J. pandurifolia's genomic DNA PCR products and cloned PCR products. Alignment of the deduced amino acid of the cloned genomic DNA PCR products of J. podagrica and J. pandurifolia to J. curcas' curcin and other RIPs revealed the presence of RIP's conserved regions such as SYFF, ALD and EAA which are the putative active sites. Genomic DNA PCR products of J. podagrica and J. pandurifolia are 674-678 bp. and 555-590 bp. respectively. Identification of the RIP genes in J. pandurifolia and J. podagrica will shed light on the medicinal properties of the ribosomal inactivating proteins (RIPs) of these plants.
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1 - Ateneo De Manila University, Department of Biology, School of Science and Engineering, Quezon City, 1108, Philippines
Ribosomal inactivating proteins.
Presentation Type: Poster:Posters for Topics
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM