Genomics / Proteomics
Grassa, Chris , Andrew, Rose , Kane , Nolan C , Rieseberg, Loren .
Linkage disequilibrium in natural populations of Helianthus petiolaris.
We used Restriction site Associated DNA (RAD) sequencing to infer genotypes at more than 70,000 loci in one hundred H. petiolaris individuals from twenty subpopulations across dune and non-dune habitats in Colorado's Great Sand Dune National Park, ten additional H. petiolaris individuals from across the species range, and ten H. annuus individuals from across the species range. We aligned RAD sequences to the draft H. annuus genome to identify marker loci and estimate the physical distance between them. We calculated measures of linkage disequilibrium for each pair of loci common to subpopulation, population, species, and interspecific groupings. This analysis has enabled the discovery of genomic regions that may differ in structure between H. annuus and H. petiolaris due to micro-chromosomal rearrangements. Additionally, RAD markers that could not be placed on the draft H. annuus genome could be tentatively located in the H. petiolaris genome based on patterns of linkage disequilibrium. Despite rapid decay of linkage disequilibrium, RAD sequencing provides a sufficient density of markers for informative analysis.
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1 - University Of British Columbia, Department Of Botany, 6270 University Blvd, Vancouver, BC, V6T 1Z4, Canada
Presentation Type: Poster:Posters for Topics
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM