Rose, Janna .
Isolation of Ellagic acid from the Bioassay-Guided Fractionation of Methanolic Crude Extracts of Rosa canina L. Galls .
During the spring of 2009, I conducted ethnobotanical fieldwork with Turkish villagers and gathered information on plant-based remedies for diarrheal diseases. After evaluating 15 of the anti-diarrheal plants for bacterial inhibition, the crude methanolic extracts of galls from Rosa canina L. (dog rose) showed the lowest MIC's (from 4 to 64ug/ml) against twelve bacterial strains that infect the human intestinal tract. Methanolic extracts of R. canina fruits were less potent (from 128 to 512ug/ml). Using an Alamar Blue Cytotoxicity Assay, I tested methanolic gall and fruit extracts for cytotoxic properties and found the extracts to have acceptable viability percentages with liver, prostate, and skin cancer cell lines. Finally, R. canina fruit and gall extracts were fractionated by high-performance liquid chromatography (HPLC) and analyzed via bioassay-guided fractionation techniques. Using Mass Spectrometry with ElectroSpray Ionization (MS-ESI) the fraction with activity was shown to contain ellagic acid. I repeated the bioassay with standard ellagic acid and obtained comparable results. Ellagic acid is a known yet under-studied phenolic compound and phytoalexin--a plant defense compound that could be used for human medicines.
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1 - Florida International University, Biological Sciences, 11200 SW 8th Street, Owa Ehan 167, Miami, FL, 33199, USA
Presentation Type: Oral Paper:Papers for Sections
Location: Lindell B/Chase Park Plaza
Date: Wednesday, July 13th, 2011
Time: 2:00 PM