Evolutionary Developmental Biology (Evo-Devo)
Vazquez, Jessica , Malcomber, Simon .
Genomic and expression analyses of THI1-like genes in plants.
Thiamine derivatives are involved in diverse biochemical pathways in plants including the Calvin cycle, citric acid cycle and glycolysis. The thiamine biosynthetic enzyme (THI1) catalyzes the condensation of thiozole and pyrimidines during the synthesis of thiamine and mutations to the Arabidopsis THI1 gene (At5g54770)lead to the production of albino leaves and premature plant death. Maize thi1-like mutants (Zmthi2/bladekiller1-R) have smaller inflorescences and leaf blade defects, but the role of THI1-like genes in other plants has yet to be investigated. A Bayesian phylogenetic analysis of 34 THI1-like genes in taxa spanning the plant clade estimates well-supported vascular plant(0.99 posterior probability [PP]), grass (1.00 PP), and eudicot (0.99 PP)clades that are generally consistent with estimated organismal relationships. THI1-like paralogs in Glycine max, Mimulus guttatus, Manihotesculentum, Zea mays and Sorghumbicolor all appear to be the product of recent polyploidization events. Genomic analyses on Medicago truncatula, Carica papaya, Oryza sativa, Brachypodiumdistachyon, and Selaginellamoellendorffii estimate THI1-like co-orthologs reside in syntenic genomic regions. Expression analyses in Medicagotruncatula, Carica papaya, Oryza sativa, and Brachypodium distachyon estimate THI1-like gene localization in vascular tissue. Together these data demonstrate the broad occurrence of THI1-like genes in plants and estimate a generally conserved role for the genes during vegetative and inflorescence development.
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1 - California State University, Long Beach, Biological Sciences, 1250 Bellflower Blvd, Long Beach, CA, 90840, USA
2 - CSU - Long Beach, 1250 Bellflower Blvd, Long Beach, CA, 90840, USA
Thiamine biosynthetic enzyme
Presentation Type: Poster:Posters for Topics
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM