Jordon-Thaden, Ingrid , Facio Viccini, Lyderson , Buggs, Richard , Chester, Michael , Veruska C. Silva, Ana , Chamala, Srikar , Davenport, Ruth , Wu, Wei , Schnable, Patrick , Barbazuk, W. Brad , Soltis, Douglas , Soltis, Pamela .
Exploring genome evolution and gene expression in natural and synthetic polyploids (Tragopogon, Asteraceae) based on growth chamber experiments.
We present an integrated study of genome evolution, gene expression, and phenotype in two recently formed allotetraploid species (Tragopogon miscellus and T. mirus), their diploid parents (T. pratensis, T. dubius, and T. porrifolius), and synthetic allotetraploids. Using SNPs identified from 454 and Illumina sequencing of the diploids, we designed primers for Sequenom Mass-ARRAY analysis. We established a growth chamber randomized block experiment for comparison of diploids and natural and synthetic allotetraploids. SNP variation between the diploid parents is used to assess homeolog loss and changes in gene expression in the allotetraploids relative to their parents and to compare broadly sampled wild individuals with synthetics. We recorded phenotypic variation in many quantitative traits and linked these data to the gene loss/gene expression data. Phenotypes scored include growth rate, physiological robustness (measured in photosynthetic efficiency), foliar anatomical variation, and reproductive timing and fitness. Karyotypes of selected polyploids were examined using FISH and GISH to investigate the role of aneuploidy and chromosomal rearrangements as drivers of homeolog loss.
Log in to add this item to your schedule
1 - University of Florida, Department of Biology, Gainesville, FL, 32611, USA
2 - Universidade Federal de Juiz de Fora, Departmento de Biologia, Instituto de Ciências Biológicas, Minas Gerais, Juiz de Fora, 36036-900, Brazil
3 - Queen Mary-University of London, The School of Biological and Chemical Sciences, Mile End Road, London, E1 4NS, UK
4 - Embrapa Coastal Tablelands, Aracaju, 49025, Brazil
5 - Iowa State University, Center for Plant Genomics, Ames, IA, 50011, USA
6 - University Of Florida, Florida Museum Of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA
Presentation Type: Oral Paper:Papers for Sections
Location: Lindell C/Chase Park Plaza
Date: Wednesday, July 13th, 2011
Time: 9:00 AM