Abstract Detail

Mycological Section

Zolj, Sanda [1], Pawley, Melissa D. [1], Ali, T'Shura S.A. [1], Goines, Jillian C. [1], Robinson, Dave [1], Lau, Joann [1].

Effect of extracts from reishi mushroom (Ganoderma lucidum) on lung cancer cell proliferation.

For centuries, Chinese herbalists have used the reishi mushroom (Ganoderma lucidum [Curtis] P. Karst) as a remedy for human diseases like hepatitis, bronchitis, and cancer. This study examined the effects of G. lucidum extract on proliferation of lung cancer. Female and male lung cancer cell lines were treated with various concentrations of G. lucidum and monitored 1, 2 and 5 days post-treatment. Overall, there was a dose-dependant, as well as time-dependent, decrease in cell proliferation. Immunoblot analysis was used to examine the levels of apoptotic and cell cycle proteins (Bcl-xL, Bcl-2, Bax, Cdc2, Cdk4,and Cyclin D) in the cell cultures following treatment. Results suggest that the decrease in cancer cell proliferation was due to signaled cell death (apoptosis). Previous studies with other cancer cell-lines have been contradictory in regards to the active component in reishi mushroom. Some reports suggest that the active component is a polysaccharide, while others point to triterpenoids. To better understand the active components responsible for cell death, triterpenoids and polysaccharides were separated from G. lucidum and further assayed in this system. Our results suggest that the triterpenoid component of G. lucidum is the bioactive component. Biochemical characterization of this ancient herbal remedy could hold promise for the treatment of lung cancer.

Broader Impacts:

1 - Bellarmine University, Biology, 2001 Newburg Road, Louisville, KY, 40205, USA

Keywords:
cancer
Ganoderma
mushroom
triterpenoids
apoptosis
cell cycle
Herbal medicine.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM
Number: PMY001
Abstract ID:430