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Abstract Detail

Genomics / Proteomics

Williams, Tanisha M. [1], Fisher, Kirsten [1], Isabel, Nathalie [2], Thompson, Stacey [2].

Identification of Populus species and hybrids through morphological analyses and single nucleotide polymorphism genotyping in the American southwest.

Poplar is a model tree with applications in biotechnology, and understanding interspecific gene flow in Populus can clarify the consequences of hybridization on native systems. Thirty years ago, a morphological analysis diagnosed hybrids between two native species (Populus fremontii and P. trichocarpa) and one European exotic (P. nigra) throughout sympatric zones in California and Nevada. These eight hybrid zones will be revisited, and the 480 samples genotyped using a 42 single nucleotide polymorphism (SNP) assay. The genotypic data will be used to assess the frequency of each species, hybrids and backcrosses, and the direction of gene flow. In addition, I will collect leaf samples and statistically analyze a series of eight measurements to determine if leaf morphology can identify Populus species and hybrids. The identities of samples based on molecular and morphological data will be compared, with the genomic identity taken as indicative. I will evaluate whether morphological characteristics of hybrids are intermediate between the pure parental species, and whether advanced generation hybrids can be identified by leaf morphology. The results of this study may increase our knowledge of the causes and consequences of gene flow in American southwest Populus, and can help focus conservation efforts to maintain biodiversity.

Broader Impacts:

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1 - California State University Los Angeles, Department of Biological Sciences, 5151 State University Drive, 350 La Kretz Building, Los Angeles, CA, 90032, USA
2 - Natural Resources Canada , Canadian Forest Service, Laurentian Forestry Centre , 1055 rue du P.E.P.S., C.P. 10380 succ. Sainte-Foy , Québec, Québec, G1V 4C7 , Canada

Population genetics.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM
Number: PGP002
Abstract ID:435

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