Espinosa-Moreno , Judith , Centurion-Hidalgo, Dora , Borges-Argaes, Rocío , Mayo-Mosqueda, Alberto .
Evaluation of potential antimicrobial activity against Staphylococcus aureus of aromatic and medicinal plants essential oils.
Food diseases are produced by the consumption of food contaminated with pathogenic bacteria and/or their toxins have been of great interest to public health. Staphylococcus aureus is a microorganism particularly virulent that causes a great variety of health conditions including pneumonia, osteomyelitis, endocarditis y bacteremia. Antimicrobial activity of essential oils obtained from dehydrated leaves of seven aromatic and medicinal plants (Ocimum micranthum, Eryngium foetidum L., Pimenta dioica (L.) Merr, Plectranthus ambonicus (Lour.) Spreng, Malvaviscus arboreus Cav., Byrsonima crassifolia (L.) Kunth y Psidium guajava L.) was evaluated against Staphylococcus aureus. Leaf essential oil was obtained through hidrodestylation in an equipment type Clevenger and the bioauthografy bioassay carried out in two TLC plaques. One of them was reveled with fosfomolibdic acid as a reference and contained positive (Amikacine 1 %) and negative (hexane) controls. Bacterial presence was observed by intense red coloration and registering the inhibition zone diameters. This chromatographic plaque was compared with the one reveled before for corroborating the presence of compounds with antimicrobial activity. Minimal Inhibitory Concentration (MIC) was determined by visual observation of turbidity presence in the microplaque wells with the addition of 100 Ã‚ÂµL 1% MTT confirming alive bacteria presence by a red coloration. Four essential oils from the evaluated species presented antimicrobial activity against S. aureus and the one of Psidium guajava L. presented the highest number of active fractions.
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1 - Universidad Juarez Autonoma de Tabasco, Division Academica de Ciencias Agropecuarias, Avenida Universidad s/n, Zona de la Cultura, Col. Magisterial, Centro, Villahermosa, Tab, 86040, Mexico
2 - Universidad Juarez Autonoma de Tabasco, Division Academica de Ciencias Agropecuarias, Avenida Universidad s/n, Zona de la Cultura, Col. Magisterial, Centro, Villahermosa, Tab, 86040, Mexico
3 - Centro de Investigacion Cientifica de Yucatan, A.C., Calle 43 No. 130, Colonia Chuburna de Hidalgo, Merida, Yuc, 97200, Mexico
Presentation Type: Oral Paper:Papers for Sections
Location: Lindell B/Chase Park Plaza
Date: Wednesday, July 13th, 2011
Time: 2:15 PM