Rein, Angela , Fishbein, Mark .
Evaluating the Utility of COSII Markers for Phylogenetic Analysis of Non-Twining Milkweed Vines (Apocynaceae, Matelea subgenus Chthamalia).
Though often common in their range throughout the Americas, the evolution, classification, and phylogeny of milkweed vines in subtribe Gonolobinae (Apocynaceae) are poorly understood, and have never received comprehensive systematic or phylogenetic study. Gonolobinae has a tropical origin, but many species exist in subtropical and temperate North America, making it an ideal group for ecological and phylogenetic study of grassland flora. Species of Matelea subg.Chthamalia are of particular interest due to variation in floral morphology and especially growth habit. It is likely that one or more losses of a climbing habit have evolved in this subgenus. Conserved Orthologous Set (COS) markers have been developed from comparative genomic data and suggested as phylogenetically useful nuclear loci. "Universal" asterid loci were screened across Apocynaceae, and milkweed specific primer sets were developed. Thirteen species of Matelea subgenus Chthamalia have been screened with three COS markers, and the resulting fragments have been sequenced, aligned, and analyzed. COS markers show at least twice as much sequence divergence when compared to chloroplast data for the same species. Preliminary phylogenetic trees that estimate relationships within the subgenus are presented. COS markers appear to have phylogenetic utility because they are more variable than plastid loci, but individual loci may not resolve many relationships. In the future, multiple unlinked loci, including COS markers, have the potential to further resolve phylogenetic relationships in Gonolobinae.
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1 - Oklahoma State University, Dept Of Botany, 104 Life Sciences East, Stillwater, OK, 74078, USA
Presentation Type: Oral Paper:Papers for Sections
Location: Lindell A/Chase Park Plaza
Date: Tuesday, July 12th, 2011
Time: 2:30 PM