Abstract Detail

Systematics Section

Lee, Chang Shook [1], Lee, Bo Ra [2], Kim, Seung-Chul [3], Lee, Namsook [2].

Phylogeny of genus Lilium (Liliaceae) based on nrDNA ITS and cpDNA matK sequences, with special emphasis on Korean species.

The genus Lilium L. includes approximately 100 species and is distributed mainly in temperate regions. About 11 taxa, three of which are endemic (L. hansonii, L. amabile, and L. callosum var. flavum), are widely distributed in the Korean peninsula. Infrageneric classification of the genus has been problematic and thus has been subject of considerable investigations. Previous molecular phylogenetic studies provided several insights into understanding infrageneric classification of the genus.These earlier studies, however, either focused on some sections (e.g., Liriotypus and Sinomartagon) or have limited sampling for the phylogeny of the entire genus. Using ITS sequences ofnrDNA, we conducted most comprehensive phylogenetic analysis of Lilium based on 196 accessions (83 species and 14 varieties)and evaluated infrageneric classification. The ITS data showed that only one section, Martagon, is monophyletic. Three sections, Archelirion, Liriotypus, and Pseudolirium are not monophyletic because two, one, and two species in each section were placed in other lineage, respectively. Two major lineages of section Leucolirion were confirmed in this study, and section Sinomartagon is highly polyphyletic. The origin of Liliumhansonii, a Korean endemic to Ulleung Island, is perplexing given the fact that it has ribotype of Martagon, while its cpDNA haplotype is similar to Sinomartagon. The origin of another endemic, L. amabile, is equally elusive. We determined that L.callosum var. flavum originated from L. callosum in southern Korea. We sequenced matK region of cpDNA, as an independent source of phylogenetic information, and reevaluated infrageneric classification of Lilium. The 50% bootstrap consensus tree is moderately resolved among major groups, while within each group is highly unresolved. Three major lineages within Lilium were identified based on matK sequences, but the origins of three Korean endemic species were not assessed due to lack of enough resolutions. Major lineages of Lilium and origins of Korean endemic species and comparisons between nrDNA and cpDNA matK gene tree will be discussed in details.

Broader Impacts:

1 - Ewha Womans University, Institute of Eco Science, Seoul, 120-750, KOREA
2 - Ewha Womans University, Life Sciences, Seoul, 120-750, KOREA
3 - Sunkyunkwan University, Biological Sciences, 300 Cheoncheon Dong, Suwon, Gyeonggi-do, 440-746, KOREA

Keywords:
Lilium
Liliaceae
nrDNA ITS
matK.

Presentation Type: Oral Paper:Papers for Sections
Session: 35
Location: Lindell C/Chase Park Plaza
Date: Tuesday, July 12th, 2011
Time: 3:15 PM
Number: 35007
Abstract ID:543