S, Deepa , Duncan , Kaylia , Annamalai , Panneerselvam , Prabhakaran, Muthuchamy , Nooruddin , Thajuddin , Muthukumaran, Gunasekaran .
Biodiversity, Antimicrobial Activity and Molecular Characterization of Actinomycetes from Salt Pan Environment.
The marine environment is an untapped source for many useful drugs and an assessment of this potential is imperative. It is well known that the antibiotics are medicinally valuable and that the actinomycetes are the potential sources of antibiotics, which could be profitably used in the pharmaceutical industries; new antibiotics to control many bacterial, fungal and neoplastic diseases of plants, animal and human beings. A perusal of the literature clearly indicates that reports on the antimicrobial activity actinomycetes from the marine environment are very scanty. The marine soil of Tamil Nadu has rich potential of microbial diversity. Neverthless, they have not been extensively explored for the registration of novel actinomycetes. In the present study soil sample were collected from salt pan region of Vedharanyam, Nagapattinam District, Tamil Nadu. The Physico - chemical parameters of soil sample were anlaysed. Totally 16 actinomycetes strains were isolated viz., Actinbispora sp., Actinobispora yunnanesis, Dactylosporangium sp., Jonesia sp., Micromonospora sp., Nocardia sp., Nocardioides sp., Saccharomonospora sp., Saccharopolyspora sp., Streptomyces albus, S. cyaneus, S. exfoliatus, S. griseoflavus, Streptoverticillium sp., S. baldaccii, Tetrrabacter sp.The isolated strains were identified based on the morphological, biochemical, and physiological characteristics. All the 16 actinomycetes were selected for antimicrobial activity. The Antibacterial activity was determined against two pathogenic bacteria such as Staphylococus aureus, Klebsiella pneumoniae: Streptomyces griseoflavus was showed maximum activity against Staphylococcus aureus and Klebsiella pneumoniae. Antifungal activity was determined against two fungal pathogenic such as Aspergillus niger, Fusarium moniliforme. Streptomyces griseoflavus was showed maximum level of inhibition against Aspergillus niger followed by Fusarium moniliforme. Molecular characterization of Streptomyces griseoflavus were evaluated by PCR amplification of 16s rDNA gene. The genomic DNA and amplified products were separated in agrose gel and the 16s rDNA gene of Streptomyces griseoflavus species isolated from soil was partially sequenced using specific 16s rDNA sequence primer. 8F:[51AGA GTT TGA TCC TGG CTC AG-31]. 1492 R: [51- ACG GCT ACC TTG TTA CGA CTT-31].
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1 - Bharathidasan University, Microbiology, School of Life sciences, Tiruchirappali, Tamil Nadu, 620 024, India
2 - Fisk University, Biology, 1000 17th Ave North, Nashville, TN, 37208, USA
3 - A.V.V.M. Sri Pushpam College , Botany and Microbiology, Poondi , Tamil Nadu, 613 503, India
Presentation Type: Poster:Posters for Sections
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM