Davoodbasha , MubarakAli , Ramasamy, Praveenkumar , Stokes , Imargobe , Thiyagarajan , Shenbagavalli , Thayalan , Mari Nivetha , Asokraja , Ilavarasi , Abdulazees , Parveez Ahamed , Nooruddin , Thajuddin , Muthukumaran, Gunasekaran .
Morphological, biochemical, molecular and antimicrobial characterization of Trichoderma viride NTDMF01.
Trichoderma viride is one of the important biocontrol fungi showing their vital role in the field of agriculture and medicine. In the present study Trichoderma viride NTDMF01 was isolated from terrestrial soil sample and characterized by morphological and biochemical methods. In order to characterize these isolate at molecular level, genomic DNA was isolated and subsequently ITS and 28S rDNA genes were amplified using specific primers and sequenced. The 28S rDNA and ITS sequences of T. viride NTDMF01 were submitted in GenBank with accession no GU585845 and HM217756 respectively. DNA barcode was developed based on the Internal Transcribed Spacer gene sequences from Barcoding Of Life Data system (BOLD) with Barcode id NTDMF001-10. Phylogenetic tree was constructed to know the taxonomic position of isolated strain. The crude extracts of T. viride NTDMF01 showed antifungal activity against various human and plant pathogens such as Trichophyton sp., Epidermophyton sp., Nigrospora sp., Aspergillus sp. etc. The active antifungal fractions were further purified and analyzed using FTIR, GC-MS, NMR for structure characterization.
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1 - Bharathidasan University, Microbiology, School of Life sciences, Tiruchirappali, Tamil Nadu, 620 024, India
2 - Fisk University, Biology, 1000 17th Ave North, Nashville, TN, 37208, USA
Presentation Type: Poster:Posters for Sections
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM