Prince, Linda .
Plastid Primers for Phylogenetics.
The availability of PCR primers for numerous plastid introns or intergenic spacer regions has increased dramatically over the past ten years, yet many botanists are still unable to confidently resolve relationships within their study group. Additionally, structural rearrangements in the chloroplast genome limit the utility of some primer combinations. The goal of the current study was to develop additional plastid markers for phylogenetic reconstruction among closely related flowering plant taxa, ideally between sister species and among subspecies. Primers were designed for 32 non-coding portions of the large single copy region of the chloroplast genome based on the complete chloroplast genomes of sixteen flowering plant species. The utility of the primers was also assessed for a variety of non-flowering land plants. Most primer pairs were tested on at least two non-model angiosperms, Monardella viminea (Lamiaceae) and Agave shawii (Agavaceae). Primer sequences, taxon specific match, and approximate amplicon size is available for review and download via the www.
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1 - Rancho Santa Ana Botanic Garden, 1500 N College Avenue, Claremont, CA, 91711-3157, USA
non-coding spacer regions
Presentation Type: Poster:Posters for Topics
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM