TED, KIM .
Variation of Inter-Simple Sequence Repeat (ISSR) Markers among the woody Sonchus alliance (Asteraceae: Sonchinae) in Madeira Variation of Inter-Simple Sequence Repeat (ISS.
For many years, plants systematics was rely on morphological characters. These days, many molecular markers are available for phylogenetic study. Since PCR (Polymerase Chain Reaction) has been invented, the window of using molecular markers in phylogenetic study has been widely opened. There are many well-known and well-established molecular markers, such as chloroplast DNA or nuclear DNA markers. Sequence data from those markers are indubitable but often variations are not sufficient enough to resolve phylogenetic relationships among closely related species or recently radiated groups. In this case, other types of markers, such as Inter-Simple Sequence Repeat Marker (ISSR) can provide further resolutions. In this research, we investigated the woody Sonchus alliance in Madeira to resolve phylogenetic relationships among taxa as well as to determine patterns of colonization within each taxon. Five taxa (including one undescribed species) are widely distributed in Madeira. Madeira is a group of young islands, located in eastern Atlantic Ocean and the estimated age of the oldest island is 20 million year. The absolute age estimation of most recent common ancestor of five taxa in Madeira is approximately 3 million years old. We estimated ISSR variations among five taxa of the woody Sonchus alliance in Madeira. Of 10 surveyed primers, we found three primers that gave us most variations and repeatable banding patterns. And from those ISSR variations, we determined phylogentic relationship among the woody Sonchus alliance in Madeira. We included three outgroup taxa from the Canary Islands: Sonchus acaulis, Sonchus congestus, and Sonchus canariensis. The ISSR variations among five tax and their phylogenetic relationships will be presented in details. In addition, the colonization patterns of Sonchus ustulatus ssp. ustulatus and ssp. maderense will be discussed.
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1 - SungKyunKwan Univ, Biology, 300 cheoncheon dong , 440746, South Korea
Presentation Type: Poster:Posters for Sections
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM