Mayfield, Dustin , Smelser, Woodson , Newton, Kathleen , Pires, Joseph .
Organellar genome sequence assembly of maize lines and grass relatives using Illumina sequence data.
The scale and economy of next-generation sequencing is greatly improving researchers' ability to investigate organellar genomes. Here, we assemble and analyze the organellar genomes of rice, sorghum, two teosintes, and other maize lines from genomic or isolated mitochondrial DNA. In the case of maize, it is thought that the species was derived from Z. mays parviglumis and that greater than ten percent of its genome was introgressed by Z. mays mexicana. Here, we show variation among these teosinte organellar genomes, along with other lines with or without expression of cytoplasmic male sterility (CMS) phenotypes. This variation may explain the molecular basis of CMS in maize. Our results demonstrate the degree that eukaryotic plant organellar genomes can be accurately assembled from shallow Illumina sequencing.
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1 - University of Missouri-Columbia, Division of Biological Sciences, 311 Life Sciences Center, 1201 Rollins Street, Columbia, MO, 65211, USA
2 - University of Missouri-Columbia, Division of Biological Sciences, 324 Tucker Hall, Columbia, MO, 65211, USA
3 - University Of Missouri, 371 B Life Sciences Center, 1201 Rollins Street, Columbia, MO, 65211-7310, USA
Presentation Type: Poster:Posters for Sections
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM