Abstract Detail

Phytochemical Section

Goyal, Shaily [1], Ramawat, Kishan Gopal [2].

Increased isoflavonoids biosynthesis in cell suspension cultures of a woody legume, Pueraria tuberosa grown in shake flasks and bioreactor.

Tubers of Pueraria tuberosa (Roxb. ex. Willd.) DC are widely used in various Ayurvedic (ancient Indian system of medicine) formulations, and contain isoflavonoids, viz., puerarin, daidzein, genistein and genistin which are under clinical trials against cancer, osteoporosis and cardiovascular diseases. Plant cell and callus cultures have always been an attractive means for the production of bioactive molecules and attempts have been made to increase their accumulation. The Pueraria cell cultures were maintained in MS medium containing 0.1 mg/l 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) and0.1 mg/l kinetin. The cells were analysed for isoflavonoids content using HPLC. Isoflavonoids accumulation in cell cultures was improved by 48 hours of elicitors' treatment at the stationary phase. Yeast extract, 150 mg/l was optimal for isoflavonoids production yielding 10 mg/l (~11-folds increase) isoflavonoids.Ethrel at 100 μM after 48 hours treatment, increased 14-folds (~12 mg/l) of isoflavonoids accumulation. Further, the addition of 1.0 mg/l 6-(γ,γ-Dimethylallylamino)purine(2iP) in maintenance medium resulted in about ~32-folds increase in the production of isoflavonoids. A marked increase in yield of isoflavonoids ~82-folds (80 mg/l) was obtained in cultures grown at 0.1 mg/l morphactin and 5.0 mg/l of 2iP. The cultures were scaled-up to 3L flasks and 2L stirred tank bioreactor. Further, the influence of angiospermic parasite, Cuscuta reflexa was studied on isoflavonoids production in cell cultures. The optimized elicitation conditions were used in vessels of varying capacity where maximum yield of ~91 mg/l of isoflavonoids was recorded in a 2 L bioreactor giving productivity of ~4 mg/l /day. The increase in isoflavonoids content was elicitor dose-dependent and can be explored to trigger high yields of isoflavonoids/secondary metabolites in production.

Broader Impacts:

1 - 500, Center Grange Road, Apt 74, Monaca, PA, 15061, USA
2 - M.L. Sukhadia University, Department of Botany, College of Science, Udaipur, Rajasthan, 313001, India

Keywords:
Isoflavonoids
Cell Cultures
Pueraria tuberosa.

Presentation Type: Oral Paper:Papers for Sections
Session: 50
Location: Lindell B/Chase Park Plaza
Date: Wednesday, July 13th, 2011
Time: 2:45 PM
Number: 50006
Abstract ID:682