Zavada, Tomas , Kesseli, Rick .
Deciphering the phylogeny of weedy/ invasive and domesticated species in the genus Cichorium (Asteraceae).
The genus Cichorium possesses two well-known cultivated species, C. endivia (endive) and Cichorium intybus (chicory). Endive is an annual, selfing species that is only known from cultivation. Chicory is a perennial, outcrossing species that was domesticated, but also recently categorized as an invasive plant taking over the US Midwest range. Cichorium pumilum is a self-compatible annual with unclear delineation within the genus. There are only a few morphological characters available for the estimation of genetic variability, thus the genetic variation within this genus is best studied with molecular markers. We have screened 41,704 ESTs derived from C. intybus and C. endivia in the Composite Genome Project database (http://compgenomics.ucdavis.edu) and identified SSRs with 10 or more repeats. Microsatellite markers were tested on 32 C. intybus individuals coming from a single population in Boston, MA and on 17 different C. intybus populations coming from the native Eurasia (6 samples per population). These markers crossamplifed on C.endivia and C. pumilum populations. The number of alleles per locus ranged from 5 to 11, with marker 6865 showing the most polymorphism. Significantly lower observed heterozygosity was detected among endive populations due to inbreeding and lower number of present alleles. Fixed heterozygosity present in several C. endivia and C. pumilum loci suggests gene duplication events. We plan to obtain C. intybus samples from US invasive ranges of chicory in order to uncover alleles possibly responsible for invasiveness, and assess the genetic diversity among chicory herbarium samples and current New England populations that might show the changes in the species genetic make-up over the past 200 years.
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1 - University Of Massachusetts, Biology, 100 Morrissey Blvd., Boston, MA, 02125, USA, Boston, MA, 02125, USA
Presentation Type: Oral Paper:Papers for Topics
Location: Portland Room/Chase Park Plaza
Date: Tuesday, July 12th, 2011
Time: 9:00 AM