Abstract Detail

Society for Economic Botany/BSA Economic Botany Section

Reese, R. [1], Wyzgoski, Faith [2], Paudel, Liladhar  [3], Rinaldi, Peter  [4], Giusti, Monica  [5], Johnson, Jodee  [6], Scheerens, Joseph [7], Bomser, Joshua [8], Hardy, James [4], Ge, Xijin  [9], Chanon, Ann M.  [7], Miller, A. Raymond  [7].

Modeling of High Field NMR Spectra of Black Raspberry (Rubusoccidentalis L.) Fruit Polyphenol Composition and the Chemopreventive Properties of the Fruit Extracts in Human Cancer Cells (HT-29).

Polyphenolic-rich black raspberry (BR) fruit extracts have shown chemoprotective activity against oral,esophageal, colonic and rectal forms of aerodigestive cancers. Several studies have identified potentially important biologically active compounds from BR, but understanding the complex interactions of constituents in the BR extracts in modulating cancer cellgrowth requires a metabolomic approach. Comparing ¹H nuclear magnetic resonance (NMR) based analyses of BR extracts with their inhibition of proliferation of HT-29 colon cancer cells, we constructed statistically based models that permitted quantitation of structure-activity relationships. Anthocyanins(cyanidin 3-rutionside, cyanidin 3-xylosylrutinoside, cyanidin 3-glucoside, cyanidin3-sambubioside, and pelargonidin 3-rutinoside) were shown to be important antiproliferative constituents of the extracts. Additionally, non-anthocyanin phenolic compounds including hydroxybenzoicacids, quercetin, myricetin, ellagic acid, and their glycosylated and methoxylated derivatives were found to be important to the overall antiproliferative activity of the fruit.

Broader Impacts:

1 - South Dakota State University, Bio/Micro, SNP 249B, Box 2140D, Brookings, SD, 57007, USA
2 - The Ohio State University-Mansfield, Department of Chemistry, 1760 University Drive, Mansfield, OH, 44906
3 - University of Akron, Department of Chemistry, Akron, OH, 44325-3601
4 - University of Akron, Department of Chemistry, Akron, OH, 44325-3601
5 - The Ohio State University, Department of Human Nutrition,, Columbus, OH, 43210
6 - The Ohio State University , Department of Human Nutrition, Columbus, OH,, 43210
7 - The Ohio State University, Ohio Agricultural Research and Development , Department of Horticulture and Crop Science, 1680 Madison Avenue, Wooster, OH, 44691
8 - The Ohio State University, ,, , Department of Human Nutrition,, Columbus, OH, 43210
9 - South Dakota State University, Department of Mathematics and Statistics, Brookings, SD, 57007
10 - The Ohio State University, Ohio Agricultural Research and Development , Department of Horticulture and Crop Science, 1680 Madison Avenue, Wooster, OH, 44691

Keywords:
black raspberry
cancer
cell proliferation
Cell Cultures
polyphenolics
anthocyanins.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:45 PM
Number: PEN023
Abstract ID:700