Drumwright, Abby , Allen, Brian , Ritchey, Patricia , Huff, Krista , Cahoon, Aubrey .
Survey and DNA Barcoding of Poaecea in Flat Rock Cedar Glades and Barrens State Natural Area, Murfreesboro, TN.
A survey of grass species from the Flat Rock Cedar Glades and Barrens State Natural Area in Murfreesboro, TN was undertaken during the 2008 growing season with the goal of creating a comprehensive list of grass species living in a single glade environment. Twenty four different species were identified during the survey. All species had been previously recorded in Tennessee but two had not been noted in cedar glades, Sporobilus clandestinus and Elymus hystrix, and one, Dichanthelium scoparium, had not been collected in Rutherford County. The survey also provides information on species distribution within the glade, amount of sunlight exposure, and flowering time within the growing season. The plastid matK and rbcL genes and the trnH-psbA intergenic spacer region were sequenced from the specimens and are catalogued in GenBank. Variance analysis of each sequenced locus was conducted with the sequences produced in this study as well as GenBank archived sequences from other grasses. Our data conservatively suggest that the CBOL recommended loci, matK and rbcL, should enable Poaceae species identification with 95% accuracy.
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1 - Pope John Paul II High School, 117 Caldwell Dr, Hendersonville, TN, 37075, USA
2 - Siegel High School, 3300 Siegel Dr., Murfreesboro, TN, 37129, USA
3 - Middle Tennessee State University, Biology, 1301 E. Main St., Box 60, Murfreesboro, TN, 37132
4 - Middle Tennessee State University, Biology, 1301 E. Main St., Box 60, Murfreesboro, TN, 37132, USA
Presentation Type: Poster:Posters for Sections
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM