Abstract Detail

Developmental and Structural Section

Ghosh , Dr. Nabarun [1], Smith, Don [2].

Fluorescent Microscopy on BSBMV infected sugar beet (Beta vulgaris) and clonal propagation via in vitro culture.

Sugar beets (Beta vulgaris L.) support the sweetener industry in the United States. Beet soil borne mosaic virus (BSBMV) is a soil borne virus and member of the genus Benyvirus that infects sugar beets. Rhizomania, caused by BNYVV (Beta Necrotic Yellow Vein Virus) is a type member of the genus Benyvirus, vectored by a fungus Polymyxa betae, and BSBMV (Beet Soil Borne Mosaic Virus), is a devastating disease of sugar beet in US responsible for the vast decline of production in the last decade. The Southwest region and Texas included in the Great Plains region are the only ones that have experienced a decline in production in the 10 years. Our approaches were: 1. Localization of viruses in the plant tissue using fluorescence microscopy and 2.Cloning and propagation for new sugar beet verities. To study the leaves and petioles with epifluorescence, thin sections were cut from the beet leaves and petioles and stored in 75-80% glycerin. Sections were observed and images were captured using a BX-51 Olympus Scope, a Digital Camera and UV source. Sections were treated with berberine hemi-sulfate and counterstained with aniline blue. Another set was stained in Fluorol yellow for possible localization of the suerbin components in the cork tissue. UV source helped in fluorescing by the lignified tissue very well under FITC. We observed the sections from the leaves and petioles at different magnifications for possible localization of viral elements. Autofluorescence was recorded in the epidermis and hypodermis region of the petiole. Sugar beet leaves emit Blue-green fluorescence that results in autofluorescence. Variation of fluorescence was recorded between the infected and non-infected regions while using the FITC filters. IAA (0.5 mg/l) and BAP (2mg/l) were used to modify the MS medium to induce dedifferentiation of somatic cells. Rooting was obtained by using IAA and IBA (0.5 mg/l) Root and shoot formation occurred directly from the explants or from regenerable callus were observed. Modification and refinement of in vitro techniques allowed us to obtain regenerable callus that had the capability of entire plant regeneration.

Broader Impacts:

1 - WEST TEXAS A&M UNIVERSITY, Life, Earth and Environmental Sciences, 2403 Russell Long Blvd., Canyon, Texas, 79015, USA
2 - University Of North Texas, 1155 Union Circle #305220, Denton, TX, 76203-5017, USA

Keywords:
Sugarbeet
Tissue Culture
Fluorescence Microscopy.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM
Number: PDS003
Abstract ID:867