Xue, Xinxin , Cronk, Quentin .
The Genetic Basis for a Red Flower Mutant in Sweet Pea (Lathyrus odoratus) .
Floral color is a trait that not only attracts pollinators, but has also attracted centuries of researchers because of its association with genetic, ecological and evolutionary questions. The Sweet pea (Lathyrus odoratus) has served as a model system for genetic studies of floral color during the early 20th century, and therefore, many of the hereditary factors responsible for a color switch from wild type to color mutants, have been identified. A single factor A is responsible for a color change from the wild type purple and blue bicolor (cultivar 'Cupani') to a red and white bicolor form (cultivar 'Painted Lady'). The genetic control of such a color switch was investigated in this study. HPLC analysis of pigment extracts indicated a major switch of enzyme flux from producing delphinidin and its derivatives to the production of cyanidin and its derivatives. However, RT-PCR showed no difference in the expression levels of f3'5'h, responsible for the switch of flux in the Anthocyanin Biosynthetic Pathway (ABP). Cloning and sequencing of f3'5'h from cDNA revealed a non-synonymous coding SNP changing the amino acid from G (Glycine) in 'Cupani' to D (Aspartic acid) in 'Painted Lady'. A co-segregation analysis is being carried out between this SNP marker with the 'Painted Lady' phenotype in the F2 generation, to determine whether there is an association between the f3'5'h allele and the red mutant phenotype. Preliminary results are presented in this poster and their significance is discussed.
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1 - University of British Columbia , Department of Botany, Biodiversity Research Centre, 2212 Main Mall , Vancouver, BC, V6T 1Z4, Canada
Presentation Type: Poster:Posters for Sections
Location: Khorassan Ballroom/Chase Park Plaza
Date: Monday, July 11th, 2011
Time: 5:30 PM